Coding
ccdB-rev
Part:BBa_K1616012
Designed by: Johanna Chesnel Group: iGEM15_IONIS_Paris (2015-09-18)
ccdB reversed
ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison.
Usage and Biology
This part is the reversed sequence of ccdB and can be used with a reversed promoter, reversed RBS and reversed terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 89
Design Notes
Illegal sites have been checked.
Source
This part is the reversed sequence of the ccdB part: BBa_P1016.
References
[edit]
Categories
Parameters
None |